The Green Lab | Field Projects International
 

There are a multitude of ways to collaborate with us at the Green Lab. To offer support in terms of an in-kind or cash donation, click the link to the right. To collaborate directly on a project you think might align with FPI’s goals, please email greenlab@fieldprojects.org. If you have read enough and are considering using the Green Lab in the future, please fill out the form to the right. We will be in touch shortly!

  • Location
  • The Field Laboratory
  • Services Provided
  • Laboratory Equipment?
  • Why Use This Lab?
 

The Amazon Rainforest, Madre De Dios Department, Peru

 

Together with our partner the Inkaterra Association (ITA), FPI is creating the first in situ molecular genetics laboratory in the heart of the Peruvian Amazon. The Green Lab is located at a brand new biological field station, the Inkaterra Guides Field Station.  

The site has several features that support this venture:

A new laboratory building

Reliable and renewable energy supply

Freezer, oven and sample storage facilities

Sterile laboratory space (a hood and autoclave)

Access to a range of rainforest habitats on site and close by

State-of-the-art technology

A strong community influence

 

At the Green Lab, we will be able to go from sample to sequence without leaving the rainforest.  Currently, this will primarily be a molecular genetics laboratory, although expansions into other areas such as tissue culture and immunoassays are future possibilities.

The Green Lab is set to launch in July 2018 with the following capabilities:

Biological sample preparation and storage, including but not limited to hair, nail, blood, faeces, serum, tissues and urine.

Kit-based DNA extraction from faeces, hair, blood (FTA cards), nail, and other tissues.

PCR-based DNA amplification with primers of your choice. We can procure these for you.

Agarose gel electrophoresis for presence/absence screening. Real-time visualisation and photography of gel results.

DNA quantification using fluorometer assays with a Quantiflor system.

Real-time portable genetic sequencing with ultra-long read lengths using MinION technology. Multiplexing possible.

Access the laboratory to perform analyses yourself, or leave it to our on-site experts.

Export of samples to an external provider for short turnaround Sanger sequencing 

Our focus is on using reliable but low-impact machinery that can be replaced easily. As such, not all of the equipment below is high throughput. Please inquire if you have a specific concern about the number of samples that can be processed at one time. This laboratory is stocked with the following equipment:

Major equipment

  • PCR machines 
  • Thermomixer
  • DNA fluorometer
  • Medium centrifuge
  • Lab computer
  • Stereoscopic microscope
  • Gel rig + visualiser
  • MinION

Supporting equipment

  • Benchtop sample cooler
  • Hood
  • Autoclave
  • Freezer/fridge
  • Pipettes and racks
  • Tube racks
  • Oven
  • Vortexer
  • Microcentrifuge
  • pH meter
  • Type I/II water purifier
  • Microwave
  • Weigh scale

Consumables (available for purchase)

  • Tips
  • PCR supplies
  • DNA extraction kits
  • Electrophoresis supplies
  • Fluorometer assays
  • Gloves
  • Tips
  • Tubes
  • Parafilm
  • Ultrapure water
  • Software: Geneious
  • MinION flowcells
  • MinION barcoding/multiplexing kit

Make history  > This will be the first in situ molecular genetics lab in the Amazon rainforest with the ability to go from sample to sequence in under 24 hours. 

Support the local economy > Ultimately, we hope that this will be a laboratory that is operated entirely by Peruvians, to serve both the local and visiting community’s needs. 

Push the envelope > Science only progresses when you think outside the bounds of traditional reasoning. Rethink your laboratory. 

Results in real time > By completing even a portion of your laboratory analyses while in the field, you can avoid future problems and refine your sample collection strategies in situ.

Stress-free sample export > Export DNA, not tissue, and save time and money.

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